Human OxHDL (Oxidized high-density lipoprotein) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9148-48, ELK9148-96, ELK9148-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Oxidized high-density lipoprotein

Assay Type: Sandwich

Sensitivity: 0.6 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Nutrition metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OxHDL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OxHDL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OxHDL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OxHDL in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only